Increased expression of TOC1 was predicted to close the feedback loop by activating the transcription of CCA1 and LHY ( Green and Tobin, 1999 Alabadí et al., 2001, 2002 Mizoguchi et al., 2002). The transcription factors CCA1 and LHY function as negative regulators of TOC1 by directly binding to the evening element (EE) in the TOC1 promoter. CCA1/LHY and TOC1 were identified as components of the feedback loop of the circadian clock of Arabidopsis ( Alabadí et al., 2001). A shared feature of circadian clocks in different organisms is that the rhythms are generated by the interactions of rhythmically expressed genes that form positive and negative feedback loops ( Wijnen and Young, 2006 Hamilton and Kay, 2008). In Arabidopsis thaliana, several components of the oscillator of the circadian clock have been identified, including the evening-phased pseudo-response regulator TIMING OF CAB EXPRESSION1 (TOC1) and two morning-expressed MYB transcription factors, CIRCADIAN CLOCK ASSOCIATED1 (CCA1) and LATE ELONGATED HYPOCOTYL (LHY) ( Schaffer et al., 1998 Wang and Tobin, 1998 Strayer et al., 2000 Locke et al., 2006 Hamilton and Kay, 2008 McClung, 2008). Biological oscillations enable plants to anticipate daily changes of the environment and synchronize plant functions with environmental cues, such as day/night cycles and temperature changes ( Green et al., 2002 Locke et al., 2005 Wijnen and Young, 2006 Gutiérrez et al., 2008 Pruneda-Paz et al., 2009).
The circadian system orchestrates diverse physiological and metabolic processes that confer a selective advantage in growth and development of many organisms, including plants ( Green et al., 2002 Michael et al., 2003 Dodd et al., 2005 Locke et al., 2005 McClung, 2008 Salomé et al., 2008 Ni et al., 2009). Results from these studies indicate that BOA is a critical component of the regulatory circuit of the circadian clock. Rhythmic expression of BOA was also affected in mutant lines of toc1-1, gi-3, and gi-4.
Rhythmic expression of other clock genes, including LHY, GIGANTEA ( GI), and TIMING OF CAB EXPRESSION1 ( TOC1), was altered in transgenic lines that overexpress BOA. In transgenic Arabidopsis lines that overexpress BOA, the period length of CCA1 rhythm was increased and the amplitude was enhanced. BOA binds to the promoter of CCA1 through newly identified promoter binding sites and activates the transcription of CCA1 in vivo and in vitro. Furthermore, the period of BOA rhythm was shortened in cca1-11, lhy-21 (for LATE ELONGATED HYPOCOTYL), and cca1-11 lhy-21 genetic backgrounds. Arabidopsis circadian clock protein CIRCADIAN CLOCK ASSOCIATED1 (CCA1) binds to the evening element of the BOA promoter and negatively regulates its expression. Transgenic lines that constitutively overexpress BOA exhibit physiological and developmental changes, including delayed flowering time and increased vegetative growth under standard growing conditions. DISC’O’CLOCK are entirely drown, designed and made in Italy.BROTHER OF LUX ARRHYTHMO (BOA) is a GARP family transcription factor in Arabidopsis thaliana and is regulated by circadian rhythms. A DISC’O’CLOCK is never the same of another! DISC’O’CLOCK are ECO-FRIENDLY products because they are created from recycling material: most of the discs with which they are produced have already exhausted its "natural function" because they are marked, scratched or otherwise no longer audible. Every DISC’O’CLOCK is decorated with a handmade processing and has the peculiarity of being produced with recycled materials, becoming a collection object because it is UNIQUE, ECO-FRIENDLY and MADE IN ITALY: DISC’O’CLOCK are UNIQUE objects because each model is produced with different disks each time. The collection DISC’O’CLOCK shows the clear connections that exist today among style, design, art, web and street is in its wholeness like a catalogue of icons of our time. So, home becomes a place to be invented by following your own inclinations and awareness and testing those elements which combine the passion for design and love for nature. It is about a design project that continuously evolves creating a big family of "Time Measures" made of signs, shapes and colors, which are obtained from the variegate catalogue of the collective memory and adapt and understand the personal taste of each person. The collection DISC’O’CLOCK has been thought for contemporary house. Pieces of furniture & Chests of drawers.
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